Using ImageJ for In-vivo RBC velocity

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Rge's picture
Using ImageJ for In-vivo RBC velocity

  Our laboratory is fairly new to ImageJ, but would like to use it to track the velocity of red blood cells during in-vivo videos of the microcirculation (mouse in-vivo microscopy of muscle/pial brain microcirculation).  

A quick summary: We inject a flourescent dye to label neutrophil (PMNs), and then capture 1 min long videos of the microcirculation (either in muscle or pial venules). These PMNs are generally moving a good deal slower than the RBCs of interest (the black background).  Does anyone have experience working with ImageJ to track this sort of background movement?

It is essential for us to be able to determine the overall flow velocity of the venules (via RBC velocity) in order to standardize our venule choice during video capture (venules of similar velocities were chosen for video capture). 

Thank you for taking the time to read and respond!  I appreciate any suggestions!