Loose-seal cell attached voltage clamp

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Ander2511's picture
Loose-seal cell attached voltage clamp


I am trying to record espontaneous activity in orgnotypic cultured slices in cell attached voltage clamp.  From looking at Perkins, K.L. (2006) J Neurosci Methods 154 (1-2): 1-18, the most appropriate method is loose seal cell attached voltage clamp.  However, I cannot find any information relating to what magnitude of seal should be used - i.e. what is the optimal seal to use when recording in the loose seal configuration? Does anyone have an opinion as to what seal resistance is the best one to use? 
Many Thanks